(Download) "Forty-Eight Hours of Biopsy Culture Improve the Sensitivity of the in Vitro Gliadin Challenge in the Diagnosis of Celiac Disease (Technical Briefs)" by Clinical Chemistry ~ eBook PDF Kindle ePub Free
eBook details
- Title: Forty-Eight Hours of Biopsy Culture Improve the Sensitivity of the in Vitro Gliadin Challenge in the Diagnosis of Celiac Disease (Technical Briefs)
- Author : Clinical Chemistry
- Release Date : January 01, 2001
- Genre: Chemistry,Books,Science & Nature,
- Pages : * pages
- Size : 191 KB
Description
Celiac disease (CD) is a long-life intolerance to gliadin in genetically susceptible individuals (1-6). Despite contrary views (7), diagnosis is still based on the histologic findings of intestinal mucosal atrophy with crypt hyperplasia in individuals on a gluten-containing diet and a return to normal after a gluten-free diet (GFD). The presence of circulating anti-endomysial antibodies (EMAs) and their disappearance after GFD confirm the diagnosis (8-10). It has recently been shown that EMAs are produced by intestinal mucosa of CD patients. EMAs disappear in treated CD patients but are newly produced after in vitro exposure of intestinal biopsy samples to gliadin (11). Culture of intestinal mucosa from treated CD patients in the presence of a peptic-tryptic (PT) digest of gliadin for 24 h frequently fails to produce detectable EMAs, but it has high specificity as well as high sensitivity in overt CD (12). Our aim was to increase the sensitivity of the in vitro culture by prolonging the duration to 48 h. We also tested a new culture method in batch to ease testing.